Autophagy‐inducing peptide increases CHO cell monoclonal antibody production in batch and fed‐batch cultures
نویسندگان
چکیده
منابع مشابه
Medium and feed optimization for fed-batch production of a monoclonal antibody in CHO cells
Background Mammalian cells are used extensively in the production of recombinant proteins, and of monoclonal antibodies (MAbs) in particular. The trend towards avoiding animal-derived components in biopharmaceutical production processes has led to the extensive use of nonanimal origin hydrolysates such as plant hydrolysates or yeast hydrolysates. The source of hydrolysates affects cell growth a...
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Most commercial media for mammalian cell culture are designed to satisfy the amino acid requirements for cell growth, but not necessarily those for recombinant protein production. In this study, we analyze the amino acid consumption pattern in naïve and recombinant Chinese hamster ovary (CHO) cell cultures. The recombinant model we chose was a CHO-S cell line engineered to produce a monoclonal ...
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Fed-batch Chinese hamster ovary (CHO) cell culture is the most commonly used process for IgG production in the biopharmaceutical industry. Amino acid and glucose consumption, cell growth, metabolism, antibody titer, and N-glycosylation patterns are always the major concerns during upstream process optimization, especially media optimization. Gaining knowledge on their interrelations could provi...
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ژورنال
عنوان ژورنال: Biotechnology and Bioengineering
سال: 2021
ISSN: 0006-3592,1097-0290
DOI: 10.1002/bit.27703